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Autoassembly protein arrays for analyzing antibody cross-reactivity.

TitleAutoassembly protein arrays for analyzing antibody cross-reactivity.
Publication TypeJournal Article
Year of Publication2011
AuthorsGaster RS, Hall DA, Wang SX
JournalNano Lett
Volume11
Issue7
Pagination2579-83
Date Published2011 Jul 13
ISSN1530-6992
KeywordsAntibodies, Antigen-Antibody Reactions, Cross Reactions, High-Throughput Screening Assays, Immunoassay, Magnetics, Nanotechnology, Protein Array Analysis
Abstract

We report an autoassembly protein array capable of rapidly screening for aberrant antibody-antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.

DOI10.1021/nl1026056
Alternate JournalNano Lett.
PubMed ID20804215
PubMed Central IDPMC3136110
Grant List1U54CA119367 / CA / NCI NIH HHS / United States
1U54CA143907 / CA / NCI NIH HHS / United States