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DNA repair and cell cycle biomarkers of radiation exposure and inflammation stress in human blood.

TitleDNA repair and cell cycle biomarkers of radiation exposure and inflammation stress in human blood.
Publication TypeJournal Article
Year of Publication2012
AuthorsBudworth H, Snijders AM, Marchetti F, Mannion B, Bhatnagar S, Kwoh E, Tan Y, Wang SX, Blakely WF, Coleman M, Peterson L, Wyrobek AJ
JournalPLoS One
Volume7
Issue11
Paginatione48619
Date Published2012
ISSN1932-6203
KeywordsAdult, Biological Markers, Cell Cycle, DNA Repair, Dose-Response Relationship, Radiation, Female, Gene Expression Regulation, Humans, Inflammation, Lipopolysaccharides, Male, Middle Aged, Phosphorylation, Predictive Value of Tests, Reproducibility of Results, RNA, Messenger, Stress, Physiological, Time Factors, Transcription, Genetic, X-Rays, Young Adult
Abstract

DNA damage and repair are hallmarks of cellular responses to ionizing radiation. We hypothesized that monitoring the expression of DNA repair-associated genes would enhance the detection of individuals exposed to radiation versus other forms of physiological stress. We employed the human blood ex vivo radiation model to investigate the expression responses of DNA repair genes in repeated blood samples from healthy, non-smoking men and women exposed to 2 Gy of X-rays in the context of inflammation stress mimicked by the bacterial endotoxin lipopolysaccharide (LPS). Radiation exposure significantly modulated the transcript expression of 12 genes of 40 tested (2.2E-06

DOI10.1371/journal.pone.0048619
Alternate JournalPLoS ONE
PubMed ID23144912
PubMed Central IDPMC3492462