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Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips.

TitleIsolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips.
Publication TypeJournal Article
Year of Publication2014
AuthorsEarhart CM, Hughes CE, Gaster RS, Ooi CChun, Wilson RJ, Zhou LY, Humke EW, Xu L, Wong DJ, Willingham SB, Schwartz EJ, Weissman IL, Jeffrey SS, Neal JW, Rohatgi R, Wakelee HA, Wang SX
JournalLab Chip
Volume14
Issue1
Pagination78-88
Date Published2014 Jan 7
ISSN1473-0189
KeywordsAntibodies, Immobilized, Antigens, Neoplasm, Carcinoma, Non-Small-Cell Lung, Cell Adhesion Molecules, Cell Line, Tumor, Cell Separation, Fluorescein-5-isothiocyanate, Humans, Keratins, Lung Neoplasms, Magnetics, Magnetite Nanoparticles, MCF-7 Cells, Microfluidic Analytical Techniques, Mutation, Neoplastic Cells, Circulating, Receptor, Epidermal Growth Factor
Abstract

Detection and characterization of circulating tumor cells (CTCs) may reveal insights into the diagnosis and treatment of malignant disease. Technologies for isolating CTCs developed thus far suffer from one or more limitations, such as low throughput, inability to release captured cells, and reliance on expensive instrumentation for enrichment or subsequent characterization. We report a continuing development of a magnetic separation device, the magnetic sifter, which is a miniature microfluidic chip with a dense array of magnetic pores. It offers high efficiency capture of tumor cells, labeled with magnetic nanoparticles, from whole blood with high throughput and efficient release of captured cells. For subsequent characterization of CTCs, an assay, using a protein chip with giant magnetoresistive nanosensors, has been implemented for mutational analysis of CTCs enriched with the magnetic sifter. The use of these magnetic technologies, which are separate devices, may lead the way to routine preparation and characterization of "liquid biopsies" from cancer patients.

DOI10.1039/c3lc50580d
Alternate JournalLab Chip
PubMed ID23969419
PubMed Central IDPMC4144998
Grant ListR33 CA138330 / CA / NCI NIH HHS / United States
R33CA138330 / CA / NCI NIH HHS / United States
U54 CA143907 / CA / NCI NIH HHS / United States
U54 CA151459 / CA / NCI NIH HHS / United States
U54CA143907 / CA / NCI NIH HHS / United States
U54CA151459 / CA / NCI NIH HHS / United States