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Matrix-insensitive protein assays push the limits of biosensors in medicine.

TitleMatrix-insensitive protein assays push the limits of biosensors in medicine.
Publication TypeJournal Article
Year of Publication2009
AuthorsGaster RS, Hall DA, Nielsen CH, Osterfeld SJ, Yu H, Mach KE, Wilson RJ, Murmann B, Liao JC, Gambhir SS, Wang SX
JournalNat Med
Volume15
Issue11
Pagination1327-32
Date Published2009 Nov
ISSN1546-170X
KeywordsAnimals, Biological Assay, Biosensing Techniques, Carcinoembryonic Antigen, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Humans, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Mice, Nanotechnology, Optics and Photonics, Proteins, Reproducibility of Results, Sensitivity and Specificity, Temperature, Time Factors, Vascular Endothelial Growth Factor A, Xenograft Model Antitumor Assays
Abstract

Advances in biosensor technologies for in vitro diagnostics have the potential to transform the practice of medicine. Despite considerable work in the biosensor field, there is still no general sensing platform that can be ubiquitously applied to detect the constellation of biomolecules in diverse clinical samples (for example, serum, urine, cell lysates or saliva) with high sensitivity and large linear dynamic range. A major limitation confounding other technologies is signal distortion that occurs in various matrices due to heterogeneity in ionic strength, pH, temperature and autofluorescence. Here we present a magnetic nanosensor technology that is matrix insensitive yet still capable of rapid, multiplex protein detection with resolution down to attomolar concentrations and extensive linear dynamic range. The matrix insensitivity of our platform to various media demonstrates that our magnetic nanosensor technology can be directly applied to a variety of settings such as molecular biology, clinical diagnostics and biodefense.

DOI10.1038/nm.2032
Alternate JournalNat. Med.
PubMed ID19820717
PubMed Central IDPMC4165514
Grant List1U54CA119367 / CA / NCI NIH HHS / United States
N44CM-2009-00011 / CM / NCI NIH HHS / United States
P50 CA114747 / CA / NCI NIH HHS / United States
P50 CA114747 / CA / NCI NIH HHS / United States
U54 CA119367 / CA / NCI NIH HHS / United States