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Microfabricated magnetic sifter for high-throughput and high-gradient magnetic separation.

TitleMicrofabricated magnetic sifter for high-throughput and high-gradient magnetic separation.
Publication TypeJournal Article
Year of Publication2009
AuthorsEarhart CM, Wilson RJ, White RL, Pourmand N, Wang SX
JournalJ Magn Magn Mater
Volume321
Issue10
Pagination1436-1439
Date Published2009 May
ISSN0304-8853
Abstract

A microfabricated magnetic sifter has been designed and fabricated for applications in biological sample preparation. The device enables high-throughput, high-gradient magnetic separation of magnetic nanoparticles by utilizing columnar fluid flow through a dense array (~5000/mm(2)) of micropatterned slots in a magnetically soft membrane. The potential of the sifter for separation of magnetic nanoparticles conjugated with capture antibodies is demonstrated through quantitative separation experiments with CD138-labelled MACS nanoparticles. Capture efficiencies ranging from 28-37% and elution efficiencies greater than 73% were measured for a single pass through the sifter.

DOI10.1016/j.jmmm.2009.02.062
Alternate JournalJ Magn Magn Mater
PubMed ID20161248
PubMed Central IDPMC2707938
Grant ListU54 CA119367 / CA / NCI NIH HHS / United States
U54 CA119367-03 / CA / NCI NIH HHS / United States