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Multiplex protein assays based on real-time magnetic nanotag sensing.

TitleMultiplex protein assays based on real-time magnetic nanotag sensing.
Publication TypeJournal Article
Year of Publication2008
AuthorsOsterfeld SJ, Yu H, Gaster RS, Caramuta S, Xu L, Han S-J, Hall DA, Wilson RJ, Sun S, White RL, Davis RW, Pourmand N, Wang SX
JournalProc Natl Acad Sci U S A
Volume105
Issue52
Pagination20637-40
Date Published2008 Dec 30
ISSN1091-6490
KeywordsBiological Assay, Humans, Magnetics, Molecular Diagnostic Techniques, Monitoring, Physiologic, Nanotechnology, Sensitivity and Specificity, Tumor Markers, Biological
Abstract

Magnetic nanotags (MNTs) are a promising alternative to fluorescent labels in biomolecular detection assays, because minute quantities of MNTs can be detected with inexpensive giant magnetoresistive (GMR) sensors, such as spin valve (SV) sensors. However, translating this promise into easy to use and multilplexed protein assays, which are highly sought after in molecular diagnostics such as cancer diagnosis and treatment monitoring, has been challenging. Here, we demonstrate multiplex protein detection of potential cancer markers at subpicomolar concentration levels and with a dynamic range of more than four decades. With the addition of nanotag amplification, the analytic sensitivity extends into the low fM concentration range. The multianalyte ability, sensitivity, scalability, and ease of use of the MNT-based protein assay technology make it a strong contender for versatile and portable molecular diagnostics in both research and clinical settings.

DOI10.1073/pnas.0810822105
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID19074273
PubMed Central IDPMC2602607
Grant List1U54CA119367-01 / CA / NCI NIH HHS / United States
N43C0-2007-00030 / / PHS HHS / United States
P01-HG000205 / HG / NHGRI NIH HHS / United States
R43AI072800 / AI / NIAID NIH HHS / United States