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Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation.

TitleNanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation.
Publication TypeJournal Article
Year of Publication2013
AuthorsKim D, Marchetti F, Chen Z, Zaric S, Wilson RJ, Hall DA, Gaster RS, Lee J-R, Wang J, Osterfeld SJ, Yu H, White RM, Blakely WF, Peterson LE, Bhatnagar S, Mannion B, Tseng S, Roth K, Coleman M, Snijders AM, Wyrobek AJ, Wang SX
JournalSci Rep
Volume3
Pagination2234
Date Published2013
ISSN2045-2322
KeywordsAnimals, Biological Markers, Biosensing Techniques, Blood Proteins, Dose-Response Relationship, Radiation, Female, Male, Membrane Proteins, Mice, Nanotechnology, Radiation, Ionizing, Radiometry, Reproducibility of Results, Serum Amyloid A Protein, Time Factors
Abstract

Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry.

DOI10.1038/srep02234
Alternate JournalSci Rep
PubMed ID23868657
PubMed Central IDPMC3715761
Grant ListR33CA138330 / CA / NCI NIH HHS / United States
U54CA143907 / CA / NCI NIH HHS / United States
U54CA151459 / CA / NCI NIH HHS / United States