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Pilot Application of Magnetic Nanoparticle-Based Biosensor for Necrotizing Enterocolitis.

TitlePilot Application of Magnetic Nanoparticle-Based Biosensor for Necrotizing Enterocolitis.
Publication TypeJournal Article
Year of Publication2015
AuthorsKim D, Fu C, Ling XB, Hu Z, Tao G, Zhao Y, Kastenberg ZJ, Sylvester KG, Wang SX
JournalJ Proteomics Bioinform
VolumeSuppl 5
Date Published2015
ISSN0974-276X
Abstract

BACKGROUND: Necrotizing Enterocolitis (NEC) is a major source of neonatal morbidity and mortality. There is an ongoing need for a sensitive diagnostic instrument to discriminate NEC from neonatal sepsis. We hypothesized that magnetic nanopartile-based biosensor analysis of gut injury-associated biomarkers would provide such an instrument.

STUDY DESIGN: We designed a magnetic multiplexed biosensor platform, allowing the parallel plasma analysis of C-reactive protein (CRP), matrix metalloproteinase-7 (MMp7), and epithelial cell adhesion molecule (EpCAM). Neonatal subjects with sepsis (n=5) or NEC (n=10) were compared to control (n=5) subjects to perform a proof of concept pilot study for the diagnosis of NEC using our ultra-sensitive biosensor platform.

RESULTS: Our multiplexed NEC magnetic nanoparticle-based biosensor platform was robust, ultrasensitive (Limit of detection LOD: CRP 0.6 pg/ml; MMp7 20 pg/ml; and EpCAM 20 pg/ml), and displayed no cross-reactivity among analyte reporting regents. To gauge the diagnostic performance, bootstrapping procedure (500 runs) was applied: MMp7 and EpCAM collectively differentiated infants with NEC from control infants with ROC AUC of 0.96, and infants with NEC from those with sepsis with ROC AUC of 1.00. The 3-marker panel comprising of EpCAM, MMp7 and CRP had a corresponding ROC AUC of 0.956 and 0.975, respectively.

CONCLUSION: The exploration of the multiplexed nano-biosensor platform shows promise to deliver an ultrasensitive instrument for the diagnosis of NEC in the clinical setting.

DOI10.4172/jpb.S5-002
Alternate JournalJ Proteomics Bioinform
PubMed ID26798207
PubMed Central IDPMC4718576
Grant ListU54 CA143907 / CA / NCI NIH HHS / United States