Title | Pilot Application of Magnetic Nanoparticle-Based Biosensor for Necrotizing Enterocolitis. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Kim D, Fu C, Ling XB, Hu Z, Tao G, Zhao Y, Kastenberg ZJ, Sylvester KG, Wang SX |
Journal | J Proteomics Bioinform |
Volume | Suppl 5 |
Date Published | 2015 |
ISSN | 0974-276X |
Abstract | BACKGROUND: Necrotizing Enterocolitis (NEC) is a major source of neonatal morbidity and mortality. There is an ongoing need for a sensitive diagnostic instrument to discriminate NEC from neonatal sepsis. We hypothesized that magnetic nanopartile-based biosensor analysis of gut injury-associated biomarkers would provide such an instrument. STUDY DESIGN: We designed a magnetic multiplexed biosensor platform, allowing the parallel plasma analysis of C-reactive protein (CRP), matrix metalloproteinase-7 (MMp7), and epithelial cell adhesion molecule (EpCAM). Neonatal subjects with sepsis (n=5) or NEC (n=10) were compared to control (n=5) subjects to perform a proof of concept pilot study for the diagnosis of NEC using our ultra-sensitive biosensor platform. RESULTS: Our multiplexed NEC magnetic nanoparticle-based biosensor platform was robust, ultrasensitive (Limit of detection LOD: CRP 0.6 pg/ml; MMp7 20 pg/ml; and EpCAM 20 pg/ml), and displayed no cross-reactivity among analyte reporting regents. To gauge the diagnostic performance, bootstrapping procedure (500 runs) was applied: MMp7 and EpCAM collectively differentiated infants with NEC from control infants with ROC AUC of 0.96, and infants with NEC from those with sepsis with ROC AUC of 1.00. The 3-marker panel comprising of EpCAM, MMp7 and CRP had a corresponding ROC AUC of 0.956 and 0.975, respectively. CONCLUSION: The exploration of the multiplexed nano-biosensor platform shows promise to deliver an ultrasensitive instrument for the diagnosis of NEC in the clinical setting. |
DOI | 10.4172/jpb.S5-002 |
Alternate Journal | J Proteomics Bioinform |
PubMed ID | 26798207 |
PubMed Central ID | PMC4718576 |
Grant List | U54 CA143907 / CA / NCI NIH HHS / United States |