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Quantification of protein interactions and solution transport using high-density GMR sensor arrays.

TitleQuantification of protein interactions and solution transport using high-density GMR sensor arrays.
Publication TypeJournal Article
Year of Publication2011
AuthorsGaster RS, Xu L, Han S-J, Wilson RJ, Hall DA, Osterfeld SJ, Yu H, Wang SX
JournalNat Nanotechnol
Volume6
Issue5
Pagination314-20
Date Published2011 May
ISSN1748-3395
KeywordsAntigen-Antibody Reactions, Antigens, Neoplasm, Biosensing Techniques, Carcinoembryonic Antigen, Cell Adhesion Molecules, Kinetics, Magnetite Nanoparticles, Models, Molecular, Protein Array Analysis, Protein Binding, Proteins, Sensitivity and Specificity, Surface Plasmon Resonance, Vascular Endothelial Growth Factor A
Abstract

Monitoring the kinetics of protein interactions on a high-density sensor array is vital to drug development and proteomic analysis. Label-free kinetic assays based on surface plasmon resonance are the current gold standard, but they have poor detection limits, suffer from non-specific binding, and are not amenable to high-throughput analyses. Here, we show that magnetically responsive nanosensors that have been scaled to over 100,000 sensors per cm² can be used to measure the binding kinetics of various proteins with high spatial and temporal resolution. We present an analytical model that describes the binding of magnetically labelled antibodies to proteins that are immobilized on the sensor surface. This model is able to quantify the kinetics of antibody-antigen binding at sensitivities as low as 20 zeptomoles of solute.

DOI10.1038/nnano.2011.45
Alternate JournalNat Nanotechnol
PubMed ID21478869
PubMed Central IDPMC3089684
Grant List1U54CA119367 / CA / NCI NIH HHS / United States
1U54CA143907 / CA / NCI NIH HHS / United States
1U54CA151459 / CA / NCI NIH HHS / United States
U54 CA119367 / CA / NCI NIH HHS / United States
U54 CA119367-05 / CA / NCI NIH HHS / United States
U54 CA151459 / CA / NCI NIH HHS / United States
U54 CA151459-01 / CA / NCI NIH HHS / United States