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Rapid Characterization of Magnetic Moment of Cells for Magnetic Separation.

TitleRapid Characterization of Magnetic Moment of Cells for Magnetic Separation.
Publication TypeJournal Article
Year of Publication2013
AuthorsOoi C, Earhart CM, Wilson RJ, Wang SX
JournalIEEE Trans Magn
Volume49
Issue7
Pagination3434-3437
Date Published2013 Jul
ISSN0018-9464
Abstract

NCI-H1650 lung cancer cell lines labeled with magnetic nanoparticles via the Epithelial Cell Adhesion Molecule (EpCAM) antigen were previously shown to be captured at high efficiencies by a microfabricated magnetic sifter. If fine control and optimization of the magnetic separation process is to be achieved, it is vital to be able to characterize the labeled cells' magnetic moment rapidly. We have thus adapted a rapid prototyping method to obtain the saturation magnetic moment of these cells. This method utilizes a cross-correlation algorithm to analyze the cells' motion in a simple fluidic channel to obtain their magnetophoretic velocity, and is effective even when the magnetic moments of cells are small. This rapid characterization is proven useful in optimizing our microfabricated magnetic sifter procedures for magnetic cell capture.

DOI10.1109/TMAG.2013.2245310
Alternate JournalIEEE Trans Magn
PubMed ID24771946
PubMed Central IDPMC3996843
Grant ListR33 CA138330 / CA / NCI NIH HHS / United States
U54 CA143907 / CA / NCI NIH HHS / United States
U54 CA151459 / CA / NCI NIH HHS / United States