Title | Sensitive giant magnetoresistive-based immunoassay for multiplex mycotoxin detection. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Mak AC, Osterfeld SJ, Yu H, Wang SX, Davis RW, Jejelowo OA, Pourmand N |
Journal | Biosens Bioelectron |
Volume | 25 |
Issue | 7 |
Pagination | 1635-9 |
Date Published | 2010 Mar 15 |
ISSN | 1873-4235 |
Keywords | Biosensing Techniques, Conductometry, Electric Impedance, Equipment Design, Equipment Failure Analysis, Food Analysis, Food Contamination, Immunoassay, Magnetics, Mycotoxins |
Abstract | Rapid and multiplexed measurement is vital in the detection of food-borne pathogens. While highly specific and sensitive, traditional immunochemical assays such as enzyme-linked immunosorbent assays (ELISAs) often require expensive read-out equipment (e.g. fluorescent labels) and lack the capability of multiplex detection. By combining the superior specificity of immunoassays with the sensitivity and simplicity of magnetic detection, we have developed a novel multiplex magnetic nanotag-based detection platform for mycotoxins that functions on a sub-picomolar concentration level. Unlike fluorescent labels, magnetic nanotags (MNTs) can be detected with inexpensive giant magnetoresistive (GMR) sensors such as spin-valve sensors. In the system presented here, each spin-valve sensor has an active area of 90 microm x 90 microm, arranged in an 8 x 8 array. Sample is added to the antibody-immobilized sensor array prior to the addition of the biotinylated detection antibody. The sensor response is recorded in real time upon the addition of streptavidin-linked MNTs on the chip. Here we demonstrate the simultaneous detection of multiple mycotoxins (aflatoxins B(1), zearalenone and HT-2) and show that a detection limit of 50 pg/mL can be achieved. |
DOI | 10.1016/j.bios.2009.11.028 |
Alternate Journal | Biosens Bioelectron |
PubMed ID | 20047828 |
PubMed Central ID | PMC2947153 |
Grant List | P01 HG000205 / HG / NHGRI NIH HHS / United States P01 HG000205-21 / HG / NHGRI NIH HHS / United States P01-HG000205 / HG / NHGRI NIH HHS / United States |