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Sensitive giant magnetoresistive-based immunoassay for multiplex mycotoxin detection.

TitleSensitive giant magnetoresistive-based immunoassay for multiplex mycotoxin detection.
Publication TypeJournal Article
Year of Publication2010
AuthorsMak AC, Osterfeld SJ, Yu H, Wang SX, Davis RW, Jejelowo OA, Pourmand N
JournalBiosens Bioelectron
Volume25
Issue7
Pagination1635-9
Date Published2010 Mar 15
ISSN1873-4235
KeywordsBiosensing Techniques, Conductometry, Electric Impedance, Equipment Design, Equipment Failure Analysis, Food Analysis, Food Contamination, Immunoassay, Magnetics, Mycotoxins
Abstract

Rapid and multiplexed measurement is vital in the detection of food-borne pathogens. While highly specific and sensitive, traditional immunochemical assays such as enzyme-linked immunosorbent assays (ELISAs) often require expensive read-out equipment (e.g. fluorescent labels) and lack the capability of multiplex detection. By combining the superior specificity of immunoassays with the sensitivity and simplicity of magnetic detection, we have developed a novel multiplex magnetic nanotag-based detection platform for mycotoxins that functions on a sub-picomolar concentration level. Unlike fluorescent labels, magnetic nanotags (MNTs) can be detected with inexpensive giant magnetoresistive (GMR) sensors such as spin-valve sensors. In the system presented here, each spin-valve sensor has an active area of 90 microm x 90 microm, arranged in an 8 x 8 array. Sample is added to the antibody-immobilized sensor array prior to the addition of the biotinylated detection antibody. The sensor response is recorded in real time upon the addition of streptavidin-linked MNTs on the chip. Here we demonstrate the simultaneous detection of multiple mycotoxins (aflatoxins B(1), zearalenone and HT-2) and show that a detection limit of 50 pg/mL can be achieved.

DOI10.1016/j.bios.2009.11.028
Alternate JournalBiosens Bioelectron
PubMed ID20047828
PubMed Central IDPMC2947153
Grant ListP01 HG000205 / HG / NHGRI NIH HHS / United States
P01 HG000205-21 / HG / NHGRI NIH HHS / United States
P01-HG000205 / HG / NHGRI NIH HHS / United States